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Dinc et al. [ 8] suggested the PDW as a new index in the diagnosis of acute appendicitis, and we verified changes in the PDW in patients with AGA.
Microarray data were validated by rigorous statistical tests; however, we verified changes by RT-qPCR of TIMP-1 and PLZF, two genes expressed abnormally in other cancers [ 44, 45].
We verified changes in microarray gene expression using quantitative real-time PCR (QRT-PCR) for representative genes from our analysis.
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Table 3 lists genes up- or down-regulated >2-fold in ofd1 MO-injected embryos (see Supplementary Material, Table S1 for the complete set) and we verified changed expression of selected genes by real-time RT PCR (Fig. 7).
However, this is one of the first studies that assessed and verified changes in methylation levels of transgenic sequences of single and stacked GM crops.
We investigated the impact of two different injection strategies on the pharmacokinetics and pharmacodynamics of insulin aspart in vivo in an open-label, two-period crossover study and verified changes in the surface-to-volume ratio ex vivo.
In supernatants of BALF, we could verify changes in the absolute concentrations and in the [local marker]/[BSA]-quotients for LBP, Hp, and Lf, which paralleled the clinical course as well as signs of local and systemic inflammation.
As a preliminary step, we verified significant changes in the expression level of Fabp7 in the COUP-TFI−/− MEF cell line by real-time RT-PCR (Figure 3A).
We verified the changes observed in lipid metabolism proteins by measuring Apo-AI and Apo-B concentrations (representing HDL-cholesterol and LDL-cholesterol respectively) by ELISA in a larger group of samples collected from malaria-infected and uninfected primigravidae and multigravidae with and without SA (primigravidae n = 150; multigravidae n = 145).
We verified that changes in mRNA expression in KO BAT correlated with protein levels.
In contrast, in our present study we verified no changes in VEGF secretion of KC keratocytes following CXL.
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