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Finally, we validated the effect of database dependency on the accuracy of KO assignment.
Next, we validated the effect of miR30a overexpression in the presence of an empty pcDNA3.1 vector under non-attachment conditions.
First, we validated the effect of sorafenib on autophagy by measuring: (1) the conversion of the cytoplasmic form of LC3 (LC3-I) to pre-autophagosomal/autophagosomal membrane-bound LC3 (LC3-II); (2) the autophagic degradation of p62; (3) electron microscopy of autophagosomes and (4) AO staining to monitor AVOs.
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In this research, we developed a surgical training simulator with virtual and haptic force feedback for maxillofacial surgery, and we validated the effects on the learning of bone-sawing skills through empirical evaluation.
In these experiments we validated the effects of RA on LAMA1 transcription levels by QRT-PCR analysis of cDNA samples prepared from RNA obtained from hMSC treated for 5 days in the presence or absence of RA.
Because the activation of EGFR and Ras is known to cause cellular proliferation in other tissues and organs, we validated the effects of the EGFR Act and Ras85D V12 transgenes in other tissues and observed proliferative responses in the wing (data not shown).
Next, we validate the effect of the local patch sizes to the probability maps by setting the parameters l of the local patch (l×l) as 3,5,7,9,⋯, and the top-ranked intensity-ordered descriptors with (K=text {int}left (frac {ltimes l-1}{2}right)+1) elements are extracted for pixel representation.
In summary, by using various in vivo models of HD we have validated the effect of several candidate genes identified through a large-scale RNAi screen for modifiers of mutant Htt aggregation.
We further validated the effect of Runx2 knockdown on cell death in another invasive breast cancer cell line, SUM-159-PT.
We therefore validated the effect of d.n.FoxO on the C26-induced increase in atrogin-1 and MuRF1 in TA muscles via qRT-PCR, and found a greater magnitude of repression than indicated on the microarray (~40% repression of atrogin-1, ~60% repression of MuRF1), both of which reached statistical significance (p < 0.05, data not shown).
We have further validated the effect of fluphenazine (Flu) in mammalian cellular and mouse models of ATD [ 22].
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