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We therefore utilised transcript profiling and pathway analysis of ICU patient skeletal muscle to generate insight into the molecular defects driving loss of muscle function and metabolic homeostasis.
For the current study we therefore utilised a mouse model of moderate pulmonary inflammation and barrier permeability induced by a low dose intratracheal LPS administration.
We therefore utilised a protocol first extracting in CHAPS to release DG from the membrane, followed by a triton extraction step to precipitate the cytoskeleton and associated proteins in the triton insoluble fraction.
We therefore utilised our FRET assay to search for interactions between two cohesin complexes.
We therefore utilised two further mitochondrial probes: MitoTracker and Mito-RFP.
We therefore utilised recombinant ligands for these receptors for comparative purposes.
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Should we therefore legalise heroin?
We therefore offer our sincerest apologies".
We therefore had to look long-term.
We therefore revolted and changed the regime.
"We therefore need support.
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