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To silence tomm-40 expression in neurons, we therefore expressed tomm-40 hairpin RNAi under the promoter of osm-6, which is specifically expressed in ciliated neurons [37].
We therefore expressed SGN3 under the control of the CASP1 promoter that shows specific expression in differentiating root endodermis, with some expression in the endodermis of the lower hypocotyl and none detectable anywhere else in the plant.
We therefore expressed one of the immunodominant, selected TCRβ sequences (TRBV31 TRBJ2-3; CAWSLGGGAETLYF) in the pTβ expression cassette, generated TCRβ transgenics, and crossed these to line 20 TCRα transgenics on the same NOD.E background.
We therefore expressed calpastatin, which is the only known specific endogenous inhibitor of calpain [54].
We therefore expressed gp150 as a fusion with GST in E.coli to look further at cell binding.
We therefore expressed and purified recombinant HrpB2XAC to perform in vitro interaction assays with HrcUXAC_207 264 and HrcUXAC_207 357 AAAH).
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We therefore express no opinion regarding the constitutionality of the search.
16 We therefore express no opinion as to whether the District Court would have had the inherent power to sanction Chambers for conduct relating to the underlying breach of contract, or whether such sanctions might implicate the concerns of Erie.
We, therefore, express treatment success as a function of closeness, betweenness, and local clustering coefficient.
We have therefore expressed a TBC domain containing fragment of the Rab1-GAP TBC1D20 (residues 1 305; TBC1D201 305) and confirmed that it possesses Rab8A-GAP activity in vitro (Fig EV3D) (Sklan et al, 2007).
We therefore stably expressed the three variants separately in HEK cells.
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