To silence tomm-40 expression in neurons, we therefore expressed tomm-40 hairpin RNAi under the promoter of osm-6, which is specifically expressed in ciliated neurons [37].
We therefore expressed SGN3 under the control of the CASP1 promoter that shows specific expression in differentiating root endodermis, with some expression in the endodermis of the lower hypocotyl and none detectable anywhere else in the plant.
We therefore expressed one of the immunodominant, selected TCRβ sequences (TRBV31 TRBJ2-3; CAWSLGGGAETLYF) in the pTβ expression cassette, generated TCRβ transgenics, and crossed these to line 20 TCRα transgenics on the same NOD.E background.
We therefore expressed calpastatin, which is the only known specific endogenous inhibitor of calpain [54].
We therefore expressed gp150 as a fusion with GST in E.coli to look further at cell binding.
We therefore expressed and purified recombinant HrpB2XAC to perform in vitro interaction assays with HrcUXAC_207 264 and HrcUXAC_207 357 AAAH).
We therefore expressed the strategy shift by the ratio of sub-strategy usage, (#W-R-W/(#W-R-W+ W-M-R-W)) for each trial.
We therefore expressed CgCDR1 and PUP1 with a strong constitutive promoter (TDH3) in the background of a CgPDR1 deletion strainto avoid interference with such factors.
We therefore expressed the extracellular domain of human Notch1 (residues 1 1735) (Figure 1A), which shares 47% sequence identity with that of the Drosophila receptor.
We therefore expressed SAF-B fusions in intact animals under the bipartite control of actin5C-gal4 and UAS-SAF-B-GFP gene expression system.
