To test the hypothesis that S-phase arrested rhesus macaque fibroblasts would reduce random integration of the targeting construct, we synchronized cells with thymidine treatment and then transfected them with a targeting construct containing the neomycin resistance gene driven by the PGK promoter.
To investigate if GSK3 is the kinase responsible for CRMP4 phosphorylation during mitosis, we synchronized HeLa cells with a double thymidine block and released them into thymidine-free media for 7.5 hours followed by a 90-minute incubation with a GSK3 inhibitor.
To this purpose, we synchronized all trials with respect to the EMG peak of the pushing subject (i.e the instant at which the sphere was released, starting to drop into the container) and kept 12 observations before this point in time.
We synchronized the kinetic data with the ultrasound images with a custom-built manual trigger apparatus which gave simultaneously a 5 V signal on the measuring computer, and it was displayed as a spike line on the ultrasound images.
As before, we synchronized TUB4-AID cells with α-factor and treated them with IAA to induce Tub4-AID degradation (Figure 5).
We synchronized the EMG recordings with video monitoring to focus on the EMG activity during the dragging events.
For this purpose we synchronized HeLa cells stably transfected with Flag-YY1 [31], using double-thymidine block, as described in the methods section.
Next, we synchronized HeLa-Flag-YY1 cells with double-thymidine, released, and harvested at 8 hours after release, with or without the addition of Cyclapolin 9 at 4 hours after release.
To explore this possibility, we synchronized U2OS cells in G1/S with a double-thymidine block, released them back into the cell cycle and monitored cell-cycle progression by FACS analysis.
We synchronized mec1 cells in G1 with the mating pheromone alpha factor, followed by release into S phase in the presence of 200 mM HU, and then collected samples at the beginning (0 hr) and after 1 hr (HU 1hr).
In this case, the solution we take is that for any two senders, if their intersection of forwarder sets is larger, we synchronize them with their forwarding nodes; if their intersection of forwarder sets is smaller, we divide the intersection into two parts and realize the synchronization of two senders respectively.
