In order to define how breast cancer cells could survive upon mitotic arrest, we first assessed Bcl-2, Bcl-xL and Mcl-1 protein expression by immunoblot analysis in Cdc20-depleted or paclitaxel-treated cells (Supplementary Figures 1C and D).
We have shown that when cells possess a greater amount of TIAF1 than Smad4, these cells survive upon challenge with TGF- β1.
Several cytokine and growth factor-based methods have been developed to expand the HSCs in vitro; however, the expansion rate is low, or the expanded cells fail to survive upon engraftment.
At medium scales, turf-banked terraces create tread-and-riser topography that is a difficult landscape for a tree seedling to survive upon because of exposure to wind, dryness, and impenetrable surfaces.
Poland, on the other hand, has been the exception, the small nation that has repeatedly imposed itself upon history, almost without meaning to do so, making desperate choices in order to survive upon a featureless plain.
Most of the crabs survive upon being returned to the sea.
Loss of dfoxO reduces the capacity to survive upon amino acid depleted diet [23] although an independent study found dfoxO null flies were insensitive to starvation when measured on the scale of days [24].
Since CFSE kinetic profiles clearly demonstrate that the disappearance rates of infected B lymphocytes is reduced upon splenectomy (Figure 2), this data indicates that these cells have a similar intrinsic capacity to survive upon short term culture but are destroyed by the immune response in vivo.
In contrast, in SiHa cells, where the activities of these enzymes are even lower than in starved HPKII cells, p53 is not induced and cells survive upon starvation, most likely due to the presence of other anti-apoptotic networks, which have emerged on the path to full transformation.
A characteristic feature of RASFs is their decreased susceptibility to apoptosis and the increased ability to survive upon certain stimulations.
