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We subsequently monitored the growth kinetics of the infection by measuring p24 production in the cell supernatants over a period of 17 days and by quantifying viral mRNA production at days 5, 10 and 15 post-infection.
We subsequently monitored ISG15 expression in cells infected with Listeria over time.
We subsequently monitored the recovery of rim fluorescence in the photobleached region.
We subsequently monitored key metabolic intermediates and their mass isotopomer distributions via LC MS/MS and GC MS.
We subsequently monitored conformational changes by HSQC spectroscopy upon titrating the domain with 14 salts: namely Na 2SO 4, NaF, NaSCN, Na 2HPO 4, NaCl, NaBr, NaNO 3, NaI, MgCl 2, KCl, CaCl 2, guanidinium chloride (GdmCl), LiF, and KCl.
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In two-pathway experiments, we first induced LTP using extracellular recording and then subsequently monitored effects of GSK-5 on the same synaptic pathways in individual CA1 pyramidal neurons using whole-cell patch-clamp recording.
By targeting the responsible cells using thermo-ablation, we could extinguish the arrhythmias in 36 of 38 patients treated and subsequently monitored using implanted devices.
In order to address this possibility, we next suppressed TIEG1 expression in WT calvarial osteoblasts using a TIEG1 specific siRNA and subsequently monitored Runx2 expression levels following 2 hours of TGFβ1 or BMP2 treatment.
We tested this hypothesis by transiently transfecting NMuMG cells with siRNAs directed against Src and subsequently monitored their ability to undergo EMT in response to TGF-β. Figure 8e shows that Src deficiency did indeed abolish the ability of TGF-β to stimulate EMT in NMuMG cells, a response reminiscent of that observed in NMuMG cells deficient in β3 integrin.
We tested this hypothesis by transiently transfecting NMuMG cells with siRNAs directed against β3 integrin and subsequently monitored their ability to undergo EMT in response to TGF-β. Figure 1c shows that β3 integrin deficiency abolished the ability of TGF-β to stimulate EMT in NMuMG cells.
To study this effect we repeated the experiments and infected Arabidopsis plants by spraying Pst DC3000 onto the leaf surface and subsequently monitored growth of Pst DC3000.
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