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So we spiked the guns of the opposition," he says with the delight of the researcher as victorious militarist.
We spiked the soil with either nanomaterial powders (using either soil or silica sand as the carrier) or aqueous nanomaterial dispersions.
Plus, we spiked our Moët with Xanax as soon as we got there, so who really knows what happened during this show.
We spiked our dataset with individuals of known ancestry genotyped at the same loci to better identify possible individuals with ancestry from Africa and South East Asia present in the INTERHEART dataset.
To rule out PCR inhibition by unknown components in the DNA extraction, we spiked the 12 samples in which no vertebrate or parasite DNA was detected with 0.2 ng of a mixture of T. cruzi and guinea pig DNAs and ran a second PCR.
To assess the potential benefits of post RT purification on qPCR inhibition, we chose a single enzyme (ACC) and performed a reaction in which we spiked the reactant cDNA product into a qPCR amplification with a known standard, 1×103 copies/µl of a purified cloned-PCR product (cytochrome b) of mammoth [36].
For a comparison of how the different RT enzyme systems would synthesize cDNA from a typical archival FFPE tissue extract, we spiked all nucleic acid extracts from a typical FFPE tissue of 1995 [30] with a known amount of RNA standard and compared their cDNA yields to those obtained from the same amount of RNA standard assayed under identical conditions, though in the absence of extract.
To confirm that this size corresponded to DNase-I we spiked NHS with radiolabeled DNase-I.
Prior to evaporation, we spiked a fraction of the samples with C-labeled standards.
As a positive control, we spiked the serum with purified exosomes from conditioned media derived from NSC34 cells.
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We spiked 73.6 ppb of Mn into the sample, measured 71.3 ± 8.7 ppb of Mn using the standard addition method.
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