We sited the gate posts from the view from the sitting room window - easily done with bamboo canes and plastic bags.
The Franklins recently asked Mr. Fields to build more amenities: a basic outhouse, shielded on three sides but open to a mountain vista on the fourth ("We sited it for the view," Mr. Franklin quipped), and a bathhouse, both built in rough-hewed pine.
We sited the vacuum chamber in a clean room, and we installed an optical fiber coupled to a visible He Ne laser, appropriate coronagraph optics, a temperature sensor and heaters in the chamber.
In Riverside, the district site was centrally located, and we sited Harvard Impactors there.
The assay allowed the quantification of methylation of the seven CpGs we sited in the HLA-G promoter, using primers designed with the Pyromark Assay Design software (Qiagen) according to the HLA-G reference sequence GenBank J03027.1.
We sited our studies at seven forest plots of the CARBONO Project plot network [ 29, 30], chosen to represent the broadest range of ant density, ant nest sizes and soil nutrient concentrations in this landscape, based on previous studies [ 11, 21].
