We observed similar effects in the transcription of the HSP70 gene, the stability of the Hsp70 mRNA and the transport and levels of HSP70 protein as we reported with the original pair of siRNAs (Pair A).
A table showing a comparison of dN/dS ratio between the values we reported with our calculation (see manuscript for methodology), versus a previously published result of a gene set (refer to reference [31] in the manuscript).
However, the favourable results we reported with metronomic cyclophosphamide probably stem from the lower-toxicity profile observed with this regimen and the fact that this therapy targets the vascular compartment instead of targeting tumour cells directly.
First, we discussed in our articles the controversial results of the literature relative to biological prognostic factors, justifying the need for meta-analyses, as we reported with K-Ras (Mascaux et al, 2005) and other biomarkers previously such as p53, bcl2, VEGF, Neu, Ki-67 or EGFR.
Recently, we reported with permeabilized and cultured mammalian cells that mannose-6-phosphate can promote LLO hydrolysis and that this reaction can deplete steady-state LLO levels if the rate of loss is greater than the rate of LLO resynthesis (Gao et al., 2011; Gao et al., 2005).
