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By direct injection of synthesized Cas9 mRNA and customized gRNA into wild-type Drosophila embryos, we previously achieved a mutational rate of up to 100% in the germline (Yu et al. 2013).
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The Ag concentrations of the prepared AgNP suspensions were higher than we had previously achieved and therefore higher than the nominal spiking concentration.
We have previously achieved more accurate threading by first aligning the interface regions as a whole via contact map representations (Fig. 1) and subsequently generating sequence profiles from the resulting alignment.
The proportion of staff vaccinated in our health service following this campaign (80.3%) was much higher than we had previously achieved, and higher than other published figures in Australian hospitals [ 17, 18].
Notably, stem cell frequency of the Procr+ basal population was calculated at one in twelve when transplanted with Matrigel [ 47]; we have previously achieved nine outgrowths from thirty-four transplants when CD24+/Low Sca-1− CD49fHigh (top 5%) c-Kit− cells were transplanted as single cells without Matrigel [ 13].
In an attempt to extend the use of multilayer coatings to photon energies higher than previously achieved, we have developed multilayers with ultra-short periods between 1 and 2 nm based on the material system WC/SiC.
Although the rearrangement of cyclic sulfites 30 and 31 overcame the need to selectively activate the secondary alcohol of 18, and provided 21 in much higher overall yield than previously achieved, we were keen to reduce both the temperature and the additional step required.
What we've shown in our work is that, despite all the variations, what comes out of the plant can be made to very high quality; in fact, the quality we reached was even higher than had been previously achieved using the CHO system.
The major reason we re-treated the patient with everolimus was the previously achieved satisfactory partial response over 2 years on initial trial.
He had previously achieved a turnaround in the food business.
(She had previously achieved a perfect score of a hundred).
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