Sentence examples for we precipitated the from inspiring English sources

We precipitated the proteins by adding 150 μL acetonitrile and vigorously shaking for 30 min, and afterward we centrifuged the samples and transferred them to autosampler vials.

We then added 100 µL of 2M NaCL with 4% PEG and incubated at 4° for 15 min. After centrifugation and extracting the aqueous layer, we precipitated the DNA with cold absolute isopropanol followed by two washes with 70% ethanol.

FMRP, by contrast, could not be detected in an identically treated control experiment conducted in parallel, in which we precipitated the presynaptic protein Syd-1GFP (Owald et al., 2010).

In order to identify the secreted factor, we precipitated the proteins in the cell culture supernatant of LiCl-treated and of non-treated cells, separated these proteins on a SDS-PAGE gel and stained the gel.

We precipitated the high-molecular-weight reaction products, removed the supernatant that contained non-integrated DNA oligonucleotides and then dried the precipitates to obtain several milligrams of each object within solid material.

Therefore, after amplification, we first precipitated the magnesium pyrophosphate to the bottom of the reaction tube by centrifugating the LAMP reaction solution for 10 seconds using a small desk-top centrifuge (6,000 rpm).

We then precipitated the 300 mM D52 eluate with ammonium sulfate and further fractionate it by Butyl Sepharose, Superose 6 gel filtration, Heparin and finally MonoQ columns to enrich for E3 ligases responsible for TBP ubiquitination (See details in the 'Materials and methods').

In order to determine if Cdc42 is activated by MapEPEC at the same time of filopodia formation, we specifically precipitated the active Cdc42-GTP form from extracts of 3T3 cells infected with E2348/69Δ map overexpressing MapEPEC using a glutathione-S-transferase (GST)–CRIB of WASP fusion protein.

In order to assess anticoccidial activity of excreted proteins we precipitated them from the supernatant and performed bioassays.

Using a GFPtrap kit, we precipitated Sti1::eGFP from the lysates of L. donovani promastigotes that coexpressed 3HA::Hsp90, 3HA::Hsp90rr, 3HA::Hsp90rr:Δ695 and 3HA::Hsp90respectivelypectively.

Some argue that by attacking Mr. Hussein, we might precipitate the very thing we are trying to prevent: his use of weapons of mass destruction.