Sentence examples for we precipitated from inspiring English sources

In the presence of proteinase K we precipitated a proteinase K-resistant fragment of 5 kDa (Fig. 2C).

In a GTPase pulldown assay, we precipitated approximately twice as much active Rac1 from Wnt4-stimulated when compared to non-stimulated cells (Fig. 1B).

In order to confirm that these CTS elements are indeed the same sequences identified by genome wide CTCF binding analyses [20], [31], we precipitated HeLa cell chromatin with an antibody directed against CTCF.

This yielded a robust Rec8-HA Co-IP signal, when we precipitated with V5-Red1.

In order to assess anticoccidial activity of excreted proteins we precipitated them from the supernatant and performed bioassays.

Using a specific antibody we precipitated σ-bound DNA from lysates of cells before and 5 min after heat shock.

Typically, we use solutions 1, 2 and 3 from any miniprep kit and rather than loading the lysate on the provided columns we precipitate 800 µL with 0.7 volume of 2-propanol.

For example, given that LGP2 can bind to stimulatory RNAs in vitro, is it possible that the complexes that we precipitate result from post-lysis 'mopping-up' of stimulatory RNA that would never in the intact cell have come into contact with MDA5?

We then precipitated myc- AMBRA1WT, and we checked for the presence of LC3-I and -II (hallmarks of autophagy).

Since all the three drugs we used precipitated in the Na-Alg solution, we decided to use it as a coating to separate the drugs from water.

To address this we sequentially precipitated Flag-HIF-1α from cells, in which we co-expressed Flag-HIF-1α, HSP70 and COMMD1-Ha.