Sentence examples for we precipitate from inspiring English sources

Typically, we use solutions 1, 2 and 3 from any miniprep kit and rather than loading the lysate on the provided columns we precipitate 800 µL with 0.7 volume of 2-propanol.

For example, given that LGP2 can bind to stimulatory RNAs in vitro, is it possible that the complexes that we precipitate result from post-lysis 'mopping-up' of stimulatory RNA that would never in the intact cell have come into contact with MDA5?

In the presence of proteinase K we precipitated a proteinase K-resistant fragment of 5 kDa (Fig. 2C).

In a GTPase pulldown assay, we precipitated approximately twice as much active Rac1 from Wnt4-stimulated when compared to non-stimulated cells (Fig. 1B).

In order to confirm that these CTS elements are indeed the same sequences identified by genome wide CTCF binding analyses [20], [31], we precipitated HeLa cell chromatin with an antibody directed against CTCF.

This yielded a robust Rec8-HA Co-IP signal, when we precipitated with V5-Red1.

In order to assess anticoccidial activity of excreted proteins we precipitated them from the supernatant and performed bioassays.

Using a specific antibody we precipitated σ-bound DNA from lysates of cells before and 5 min after heat shock.

We precipitated the proteins by adding 150 μL acetonitrile and vigorously shaking for 30 min, and afterward we centrifuged the samples and transferred them to autosampler vials.

Using a GFPtrap kit, we precipitated Sti1::eGFP from the lysates of L. donovani promastigotes that coexpressed 3HA::Hsp90, 3HA::Hsp90rr, 3HA::Hsp90rr:Δ695 and 3HA::Hsp90respectivelypectively.

We precipitated Bub1 or Knl1 (Vleugel et al., 2013) from mitotic lysates of HeLa cells treated with or without the Mps1 inhibitor Reversine (Santaguida et al., 2010).