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Power law distribution of node degree, one of most important network topological characteristics, was analyzed as we performed previously [ 14].
Furthermore, we analysed data of a PCa meta-analysis study which we performed previously (Massoner et al, 2013).
To identify changes in gene expression for thermal adaptation, four Escherichia coli strains were acquired during experimental evolution that we performed previously [ 5].
The log odds appears useful for integrating node-negative and node-positive cases while avoiding more complex modeling, which we performed previously [ 39].
These migration assays are more quantitative and controlled than the scratch assay we performed previously, and we feel that the use of breast cancer cell lines for migration analysis is particularly relevant, given the roles for EMT in breast cancer.
Heterodimeric TALENs were targeted to the homeobox region of the ey gene as was the CRISPR/Cas9-mediated ey gene disruption we performed previously (Nakanishi et al., 2014), allowing us to compare the efficiency of the TALENs and the CRISPR/Cas9 system to introduce mutations (Table 3).
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This analysis will continue on an analysis that we have performed previously [ 55].
Cell lysates were then assessed for Rac activity using a Pak1 Rac Binding Domain assay, as we have performed previously [ 21, 23].
For detection of MCPyV we performed a previously established PCR assay using primers situated outside of the helicase domain and generating an amplicon of 138 bp [30].
We performed immunocytochemistry as previously described (Fritsche et al. 2005; Moors et al. 2007).
Additionally, we performed qPCR for previously identified, commonly used housekeeping genes ACTB, GAPDH, HPRT1, and TBP.
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