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Whilst EGFR and Sprouty2 still bind to c-Cbl TKB domain in the presence of additional serine phosphorylation, these binding affinities are weaker than we originally detected with the single tyrosine phosphorylated peptides [14], and may indicate a weak association with c-Cbl in cells.
We originally detected ALKBH3 expression in the prostate, and were able to demonstrate that a number of molecules associated with ALKBH3 were involved in cancer metastasis or resistance to anticancer drugs.
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Although we lost the CTCs of two samples during the DEPArray™ procedure (as expected from the transfer efficiency), we found more CTC events than originally detected with CellSearch® in three cases.
After fitting the model to the 14 649 positions, we evaluated the performance using 497 candidate mutations originally detected using SNVMix1 at 10×, which were validated using Sanger amplicon sequencing (Section 2).
Based on a similar assumption for this outbreak (56 cases originally detected = 40% of the outbreak), we might expect to find about 140 patients with LD.
However, our hypothesis is that the tumor causing death may in fact represent the original primary tumor which could not be originally detected with the available imaging methods.
In the other 5 of 20 patients, the same mutations as those originally detected (identical nucleotide exchange) reappeared and became detectable by Sanger-sequencing between 1.7 and 5.5 years after last detection (median 4.4 years; Table 1, patients 16 20).
To further confirm the reliability of our model, we reproduced the same mutations in EML4-ALK, where they were originally detected.
We did not count these perfectly correlated variables as false positives, and counted them as a detection if the causal locus was not originally detected.
Furthermore, we have used the advanced intercross lines to give more support to Vra2, originally detected as a suggestive QTL.
This resulted in the detection of 18,173 SNPs indicating that 13% of the SNPs originally detected either ssp.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com