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Compared to our published annotation pipeline, where we only validated and updated known gene models, which are mostly protein-coding, the new implementation is more inclusive in annotating both coding and long non-coding RNAs.
Though we only validated up-regulated genes, we believe these results can support the validity of down-regulated genes of our microarray data.
We only validated our previous decision.
Although we only validated these genes on 3 samples, the unexpectedly high concordance rate between the results of rat and human skin explant assays strongly suggests that the rat skin explant assay is an informative model for human GVHR and possibly GVHD.
For the small CNVs, we only validated the sites that involved known genes.
We only validated the result of RT-PCR using FISH or iAEP IHC using commercially available antibody in a limited number of patients.
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We only considered validated episodes and not visits; furthermore, multiple examinations within 30 days of the first reported diagnosis were considered as being related to just one episode (although these were reported very rarely).
We only cross-validated eight instruments in the document analysis from which low levels of agreement at the sub-category level were initially attained.
We recovered from CPI the only validated therapeutic target of AD, acetylcholinesterase (ACHE), and highlighted several other putative targets.
As well as rerunning our final model including only validated myocardial infarction events, we carried out several other sensitivity analyses, modifying the final model.
Although they are not validated in children, we explored their utility in comparison with the PVAS, the only validated vasculitis activity score for children.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com