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For example, inverse PCR from both 5' and 3' ends of C32 produce a single unique product and we only recovered one insertion in plasmid rescue experiments.
Intriguingly, in S. lemnae we only recovered molecules with permutations (Figure 5) or large deletions (lines marked Δ in Figure 3; detail in Figure S2B) in the middle-late or late stage of polytene chromosome formation.
The growth rate of the bacteria was unaffected when overexpressing elaD, but we only recovered about 50% of the wildtype protein when compared to the amounts of C313S mutant (Figure 4A).
In contrast we only recovered 1.5% (2/137) Upf2fl/fl; Alfp-Cre P0-P2 newborns demonstrating that liver-specific loss of UPF2, and by inference NMD, leads to perinatal lethality (Figure 1C).
To incorporate heterologous information, we only recovered potential gene coding sequences.
Interestingly, for glyco-variant N158D and N198D, we only recovered 50% of the enzyme in total, which we also found in the flow-through.
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"We don't train, we only recover so that's the situation now," he said.
Asked whether it might be his training system that could be to blame, the manager appeared to bridle, saying: "We don't train, we only recover so that's the situation now".
"Maybe I could look at our training methods but we don't train - we only recover.
Since we only recover large amounts of mutant RNA from the chromatin fraction, we hypothesised that degradation was most likely to occur at the chromatin or otherwise very soon after release into the nucleoplasm.
"We know that there are 30 dead, but so far we have only recovered seven bodies," said Gen. Fernando Tapias, Colombia's army chief.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com