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To investigate whether AP180 resides in the bladder, we observed live cells under hyposmotic conditions (Fig. 2B) and localized AP180 with antibodies against GFP and CaM in fixed cells (see Fig. S1).
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Furthermore, we observed living larval body wall muscles in tw mutant flies by using the MHC-tauGFP reporter, a muscle marker (Figs. 3F H).
As expected, we observed living cells exhibiting moderate to high P-gp expression and relatively low DNR uptake.
Many new Muscovites were still peasants, he observed, living village lives according to village rhythms.
"Cells were observed living or fixed in OsO4 vapours using Nomarski Interference microscopy.
In this paper, observed living arrangements will be compared to expectations computed using micro-simulations and analytical models.
Neither Haliclona cymaeformis or Ceratodictyon spongiosum have been observed living without the other partner [ 52].
In the control group we can observe living cells with nuclei well formed and adhered to the blade [ 26].
Shepherd saw this loss beginning more than 60 years ago, and her book both mourns it and warns against it: "This is the innocence we have lost," she observes, "living in one sense at a time to live all the way through". Her book is a wry, beautiful hymn to "living all the way through".
Cells were then observed live, in real-time, using a temperature controlled confocal microscope imaging system.
Cells were observed live with fluorescence microscopy at a wavelength of 543 nM.
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