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We next adopted these constructs to meet our specific purposes.
We next adopted a similar strategy in the wing imaginal disc and tested Hh-N range of action.
We next adopted the same terminator mapping strategy for four other CLIP-seq positive genes (AKIRIN1, PTCH2, THOC2, and WDR13).
As a complementary approach to gene silencing, we next adopted overexpression of GFP-Pfn1 in MDA-MB-231 cells as previously accomplished in BT474 breast cancer cells (Roy and Jacobson, 2004).
To further investigate the involvement of p38-MAPK in the ZOL resistance mechanism, we next adopted a pharmacological approach to inhibit p38-MAPK activity using the specific inhibitor SB203580 (SB).
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Some elections officials next adopted lever machines, which record each vote mechanically.
But the "mysterious element" was also present in the method Freud next adopted — of pressing the patient's forehead and urging him to remember — as it was in the culminating free-association method of psychoanalysis proper.
Under Massachusetts's influence, the Congress next adopted the Suffolk Resolves, recently voted in Suffolk county, Massachusetts, which for the first time put natural rights into the official colonial argument (hitherto all remonstrances had been based on common law and constitutional rights).
However, none of them can guarantee the nonnegativity of A. In order to impose the nonnegativity constraint on A, we resort to use a square change of variable which was introduced by Chu et al. [50] for NMF, next adopted by Royer et al. for NTF [37] and by Coloigner et al. for semi-nonnegative INDSCAL [40]: A = B ⊡ B = B ⊡ 2 (10).
First, audio signals are all segmented into fixed-length frames which may cause serious synchronization problems under cropping, adding, and time stretching; next, adopted features are not suitable enough to characterize the content of music signals; last, all algorithms take a yes/no decision instead of a fuzzy one.
Classical and quantitative real-time RT PCR approaches were next adopted to evaluate DKK1 expression in normal tissues.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com