In the first experiment, we made cell-body specific lesions in the rat SLD, PMnR and PCRt and then recorded the EEG, neck electomyogram (EMG), masseter EMG and electrooculogram (EOG).
In evaluating the specificity of the CHD5 antibody, we made cell pellets of ZR-75-1 (CHD5-negative) and T-47D (CHD5-positive) cells in agarose, fixed them in formalin, embedded in paraffin, prepared sections, and then conducted IHC staining.
There is a strong presumption that these changes should also be apparent in extracellular recordings, but to check this explicitly, we made cell attached recordings from visually identified, layer 5, pyramidal neurons in brain slices prepared from young mice, during epileptiform events induced by removing Mg2+ ions from the bathing medium (0 modelmodel; Supplementary Fig. 4).
We found that rox3Δ alone made cells more resistant to TBZ, spt2Δ, or pds5Δ made cells more sensitive to TBZ, and not3Δ showed no significant effect.
To compare PRE and SEN cells, we made PRE cells quiescent by culturing to confluence, thereby controlling for effects of cell proliferation (Table 1).
To investigate the function of SMAD4 in PDAC cells, we made a cell line in which SMAD4 was stably knocked down by performing lentiviral transduction with shRNA against SMAD4 in SMAD4-positive PANC-1 cells, as well as a control cell line using a control construct.
To determine whether this observed defect in neural differentiation is due to any one of these Mecp genes, or is indicative of functional redundancy between them, we made NS cells lacking each gene individually, i.e. Mecp2−/y NS cells, Mbd2−/− NS cells, and Zbtb33−/y NS cells.
To examine the synaptic processes that mediate directional signaling in the starburst amacrine cell (SBAC), we made whole-cell voltage-clamp and current-clamp recordings from ON SBACs in isolated whole-mount rabbit retina, and stimulated the retinal circuitry with patterns of light projected onto the retinal photoreceptors (see methods).
We made bottom cell current limited tandem cells on Asahi U-type substrates, and they showed an initial efficiency of 10.2% (Voc = 1.34 V, FF = 0.69).
We made ES cells from Mll2F/F; Rosa26-CreERT2/+ blastocysts.
