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Using immunohistochemistry and confocal laser microscopy, we localised the site of expression of the translated PTGER4 transgene to the plasma membrane compartment (Figure 2B).
To determine the sub-cellular distribution of SpARC1 in more detail, we localised the expressed protein in oocytes and eggs by immunocytochemistry (Fig. 5).
Here, we describe a ChR2 construct that we localised specifically to the AIS by adding the ankyrinG-binding loop of voltage-gated sodium channels (NavII-III) to its intracellular terminus.
By deficiency mapping, we localised hau to the cytological interval between 83B7 and 83B8 uncovered by the deficiency Df 3R ED5187 on the right arm of chromosome 3 (Figure 11A), and gho to the cytological interval between 22D4 and 22D6 defined by the overlapping region of the deficiencies Df 2L Excel7010 and Df 2L BSC688 on the left arm of chromosome 2 (Figure 11C).
Within this region, we localised the promoter of the cyclin T1 gene, an ubiquitously expressed gene.
We localised LMO4 and LDBs in tissue sections by immunostaining using specific antibodies.
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initialisation: using pictorial structure type models as in [7], we localise all the targets along with the configuration of their parts, for iterations, (a) evolve particles using constrained genetic operators as in EPMCMC filter, (b) the expected likelihood data association provides an efficient solution for data association of each object into the particle weights of the EPMCMC filter. .
Using live cell imaging and reverse genetics in the rodent malaria model P. berghei, we localise two unique IMC sub-compartment proteins (ISPs) and examine their role in defining apical polarity during zygote (ookinete) development.
I strongly believe we need localised and community approach and that is why localism matters in the present climate.
But we think localised good news stories are not enough, and that the reality of 30 years of uninterrupted union decline must be faced.
We have localised retention time areas both in the chromatographic peak and the UCM data that strongly influence hydrate plug inhibition in some of the oils from the sample set.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com