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After the reconstruction detailed in File S1, we have reduced restriction site overlap between the five markers and the pBluescript/pBluescript II MCS to the point where 16 of the 18 common restriction sites in the polylinker region of our pRSII plasmid series are universal; EcoRV and BstXI, one or both of which occur in all five mutagenized markers, were the only sites we left intact (Table 1).
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We leave intact the moral foundation vital to me as a moral artist and to Mom as a moral being.
Although the SB repeats were left intact, we only chose to use the PB to generate mutated libraries in a range of human cell lines, due to its higher efficiency and lower insertion site bias compared with SB [ 13, 26].
Fortunately, the 60 million pages scanned to date were to be left intact, so we all breathed a collective sigh of relief and kept on Googling.
"We are not used to seeing empty buildings left intact.
We nonetheless concede the possibility that critical elements are left intact.
We therefore performed punctures under two conditions: with the skin opened beforehand and with the skin left intact.
Dura was left intact.
Lesser charges were left intact.
The art is left intact.
The party was left intact".
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com