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We initially ascertained whether tissue histamine synthesis is altered in WT mice after sepsis induction by CLP.
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Probands were initially ascertained at random from the tribal register and the families of alcoholic probands were extended, largely coalescing into one pedigree.
For a subset of the WHS women (those initially ascertained through the Mayo Clinic), the underlying cause of death was determined through the Social Security Death Index.
Sequences were aligned and analyzed with ChromasPro 1.34 (Technelysium Pty Ltd), and nucleotide mutations were initially ascertained relative to the revised Cambridge Reference Sequence (rCRS) [ 79].
Cancer outcomes were initially ascertained through either self-administered questionnaires at semi-annual visits (CTs) or through questionnaires that were mailed annually (OS).
Manganese concentrations were initially ascertained for a subset of 1,508 wells, for the purposes of other studies nested within the parent cohort.
In conjunction with the 1994 data collection phase, we obtained either a questionnaire (including proxy questionnaires) or a death certificate for 6495 (84%) of the 7758 women initially ascertained for the WHS and Dieckmann studies.
Deaths that occurred during the follow-up period were initially ascertained either through direct contact by relatives on receipt of the postal questionnaire or, if this was not returned, by further enquiry made to ascertain the subject's vital status.
All neutrality tests were carried out using data obtained with an initially ascertained sample of 80 chromosomes, except in the Fay and Wu test, where an extended sample of 80 genotyped individuals was used.
Finally, it appears to be worth pointing out that methylation analysis invariably revealed hypermethylated DMR(s) in all the 26 patients who were initially ascertained because of bell-shaped thorax with coat-hanger appearance of the ribs.
We initially investigated whether cluster residency status could be ascertained reliably from a written description of household location.
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