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In a transient expression condition, we have predetermined the optimum transfection conditions [DNA concentration (total 10 µg/10-cm culture dish), molar ratios (H∶L = 1∶1 or 1∶2) (data not shown), use of lipofectamine (LF -LTX reagent, and CHO-K1 ceLF -LTXng eight differeagentst cells tested].
"Otherwise the court will have predetermined the winner of the midterm elections," Mr. Schumer said.
The expedition's other principal is Dr. Thomas Potter, a physician convinced that races have predetermined character types.
(Though the Taliban do frequently hold trials, they generally have predetermined outcomes, and the militants are widely documented as having conducted executions with no trial at all. Still, they are often reluctant to admit or discuss killing women).
As personalization becomes more pervasive, however, it has the potential to invisibly channel consumers who might mistakenly think they are browsing freely, limiting their experience to the views algorithms have predetermined they might like and act upon.
Some people have predetermined feelings of belonging and a favorite party; ultimately, people care about what their president will do for them on a personal level.
Motivated hiring managers can sometimes make a difference, but most firms have predetermined policies regarding applications from those without permanent residency.
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Ha Thuy Vy
MA of Applied Linguistic, Maquarie University, Australia