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The immunogold and immunofluorescent staining, as well as western blot analysis presented in this manuscript showed that the antibody we generated did indeed bind predominantly to podocytes.
Nevertheless, under the conditions used, the anti-SVSP antibodies that we generated did not allow us to confirm that SVSPs are indeed secreted into the host-cell cytoplasm and translocate to the host cell nucleus.
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The sequence we generated does not exactly match that of L. donovani.
For three hypothetical populations with different levels of clustering, we generated DO and DS population size estimates for a range of detection probabilities and survey proportions.
This involved most of the junior crew members, all of the protected species observers (here to make sure the sounds we generate do not unduly affect the marine mammals) and all of the science party except for myself.
Nevertheless, the range in body size we generated experimentally did not differ significantly from the range in size in field-caught beetles (see Figure 1 figure supplement 2).
Thus, we generated mice that do not differ in testosterone levels across genotype within sex.
To attempt to eliminate the former, we conducted a secondary screen in which we generated mosaic eyes, but did not induce ablation of the sec5 ts tissue.
To do so, we generated a phantom with 5 × more counts by combining the data from all breathing phases.
To do this we generated Gab2 mutant mice with heterozygous and homozygous deletions of STAT5.
To do this we generated phylogenetically independent contrasts [28] for the various traits.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com