Sentence examples for we generated against from inspiring English sources

In addition, we used a polyclonal antibody (S1337) that we generated against a region located at the carboxy-terminal end of human and mouse Kaiso (immunogenic peptide: NVTDGSTEFEFIIPESY, AA 655 671) [23].

We could not detect endogenous HIC protein in any of these cell lines by Western blots using a rabbit polyclonal antibody that we generated against HIC (not shown and Fig. 1B, first lane).

Antibodies we generated against the mouse FLJ46154 protein recognize a protein of the predicted size for FLJ46154 in mouse brain extract (supplementary figure 6).

Importantly, by using a commercial USP7 antibody and a rabbit antiserum that we generated against USP11, it was possible to confirm that the endogenous USP7 and USP11 in 293T cells co-precipitated with Flag-tagged PRC1 proteins.

We generated all-against-all bidirectional BLASTP alignments between L. hokkaidonensis LOOC260T and each reference strain.

Therefore, we generated antibodies against these potential 20 new amino acids of the human sequence.

We generated antibodies against the 52 kDa and 63 kDa globulins and investigated protein expression patterns by western blotting.

We generated antibodies against lamprey visual arrestin and β-arrestin.

To perform these experiments we generated antisera against Drosophila Drp1 and dMfn using peptide immunogens corresponding to sequences in these proteins.

To examine the interaction between Ybp2 and known kinetochore proteins, we generated antibodies against Ybp2 and strains that express several myc-tagged kinetochore proteins under their own promoters.

To assess the dynamics of primary transcription taking place in the nucleus, we generated probes against intron 2 of rho and epsilon, and performed whole mount in situ with pre-circulation stage embryos.