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If we further fix and let, then.
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It can be mentioned that we also further fixed the value of D B to the average value, i.e., 1.9 × 10−12 m/s; however, the linearity of the values of μ s ' did not improve any more.
They can further fix an appointment for rectifying the problems too.
Finally, 2% formalin/PBS solution was used again to further fix the cells.
The brains were further fixed in fresh fixative for 24 h at 4 °C.
The cerebellum was dissected out, further fixed in the same fixative using microwave irradiation (PELCO 3451 laboratory microwave system; Ted Pella), then dehydrated up to 70% ethanol and embedded in LRWhite resin (medium grade, SPI).
Dissected striatum were further fixed with the same fixative for 2 4 h at 4 °C and postfixed with 2% OsO4 in buffer and embedded in Araldite.
Tissues were harvested and further fixed with the same fixative for at least 4 h, followed by treatment with 15% sucrose in PBS for 4 h and then with 30% sucrose solution overnight.
The brain was dissected out and further fixed by immersion in Bouin fixative for 2 days.
Briefly, the fixed tissues were trimmed and further fixed for 24 hrs.
Tumors were excised from animals and further fixed by immersion in the same fixative, dehydrated and embedded in paraffin wax.
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