Sentence examples for we fractionated the from inspiring English sources

We fractionated the nsph-CM into fraction A (>30 kDa) and fraction B (<30 kDa; Fig. 1B) and found that in low density cultures fraction A has nsph stimulatory activity approximating that of whole nsph-CM, and fraction B stimulated nsph formation by 1.5-fold (Fig. 1C).

To characterize the immune response to Acai polysaccharides, we fractionated the crude polysaccharide preparation and tested these fractions for activity in human PBMC cultures.

Toward this goal, we fractionated the cytoplasms of SW620 cells into 12 fractions by 8.5 60% sucrose gradient.

To investigate the formation of insoluble FN matrix in these cells, we fractionated the cell layers into deoxycholate-soluble and insoluble fractions and analyzed the material by non-reducing Western blotting to visualize FN stabilized via cysteine-bridges.

To assess whether the aggregates (identified by SLS) resulting from the interaction between PrP and AChE contained both proteins, we fractionated the solutions into supernatant (S) and pellet (P) fractions by ultracentrifugation and separated them by SDS-PAGE.

To further examine this redistribution of LRP6 towards clathrin by Dab2, we fractionated the lipid raft (caveolin-containing) and nonlipid raft (clathrin-containing) fractions by sucrose density gradient centrifugation.

To confirm that the prominent monosomal peak is reflective of ESC biology and not of poor ribosome fractionation, we also fractionated the ribosomes of neuroprogenitor cells derived from the ESC.

We further fractionated the hydrophobic IPC effluent into four fractions with particulate size > or <10 or 30 kDa.

As crude methanol extract of A. macrocephala has been reported for antispasmodic activity, hence, we currently fractionated the methanol extract to know in which fraction(s) the relaxant constituent(s) concentrate.

We then fractionated the cells into cytoplasmic soluble, nuclear soluble and cytoskeletal/matrix-associated insoluble fractions.

DOI: http://dx.doi.org/10.7554/eLife.08536.006 To purify E3 ligases, we first fractionated the S100 lysate on an ion exchange column (D52) into multiple fractions: flowthrough, proteins bound to the column and eluted at 0.2 M KCl, 0.3 M KCl and 0.5 M KCl, respectively.