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To identify the source of testis S-2HG, we fractionated testis extracts and identified the fractions that actively produced S-2HG.
We fractionated mouse brains using an iodixanol gradient fractionation system identical to that described in the published cell culture study [4] (Fig. 5).
After treatment with individual PCB congeners for 5 min, we fractionated Caco-2 cells to isolated membrane fraction.
To characterize the immune response to Acai polysaccharides, we fractionated the crude polysaccharide preparation and tested these fractions for activity in human PBMC cultures.
To determine whether the endogenous Tmub1/HOPS is localized in the membranous compartment, we fractionated mouse brain extracts into cytosolic and membranous fractions (Figure 2F).
To determine the subcellular distribution of STIM1, we fractionated rat brain by differential and density gradient fractionation methods as previously described [25], [26].
Toward this goal, we fractionated the cytoplasms of SW620 cells into 12 fractions by 8.5 60% sucrose gradient.
To confirm the presence of MYOC aggregates in the transgenic flies, we fractionated homogenates from fly heads into soluble and insoluble fractions.
To investigate the formation of insoluble FN matrix in these cells, we fractionated the cell layers into deoxycholate-soluble and insoluble fractions and analyzed the material by non-reducing Western blotting to visualize FN stabilized via cysteine-bridges.
We fractionated 3T3-L1 cells into nuclear extracts and cytoplasmic S100 fraction, and we found that a large amount of TRIM23 was distributed in the cytoplasm.
To assess whether the aggregates (identified by SLS) resulting from the interaction between PrP and AChE contained both proteins, we fractionated the solutions into supernatant (S) and pellet (P) fractions by ultracentrifugation and separated them by SDS-PAGE.
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