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Since the annotated pathways of arachidonic, linoleic and α-linolenic acid as well as the biosynthesis of unsaturated fatty acids are interrelated and comprise a high number of metabolites with altered intensities (Table 1) we focussed in more detail on the metabolite ion masses of those pathways.
For these reasons, we focussed in this study on the WGS sequences from HGP: one feature of this is that sequences are in their pre-assembled state, avoiding certain artefacts introduced during the assembly process.
Among the 21 genes with ≥ 4 fold expressional change (table 1), we focussed in our validation experiments on 5 genes, that represent - in our eyes - genes of special interest: the transcription factors (TFs) EGR1 and ATF3, the ER-stress response gene PPP1R15A, the pro-apoptotic mitochondrial protein PMAIP1 and the E3 ubiquitin ligase TRAF6.
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That's part of the reason we focused in our article on the research of Jennifer A. Francis of Rutgers University.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com