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We first stimulated cells with a long but very dim light pulse (0.01 mW, 1000 ms).
To test this hypothesis, we first stimulated cells with unlabeled insulin for two days, then added labeled insulin.
To begin to address the hypothesis that high antigen loads may drive this population, we first stimulated PBMC from healthy donors with a set of different recall antigens, mitogens and superantigen (Figure 6A).
Focusing on melanoma cells and on the "cytokine-like" properties of IL-24, we first stimulated a panel of 14 different cell lines with commercially available recombinant IL-24.
To examine whether different TLR2 ligands activate cells in ligand-specific ways, we first stimulated the murine macrophage/monocyte cell line, Raw 264.7, for 0, 5, 15, 30, 45, or 60 minutes with each of the TLR2 ligands: LTA (TLR2/6/CD36), R-FSL1 (TLR2/6/CD36), S-FSL1 (TLR2/6), Pam2CSK4 (TLR2/6), or Pam3CSK4 (TLR2/1).
To focus specifically on synaptic interactions and exclude all other uncertainties associated with the estimation process, we first stimulated the neuron with excitatory and inhibitory inputs alone (Fig. 4 A and B, red and blue traces respectively) and then with both inputs activated simultaneously (Fig. 4 A and B, black).
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Differentiated HL-60 cells were first stimulated with a low dose of fMLP before we triggered iRap induced Rac activation.
We also thank Pedro Curi Hallal for valuable inputs on earlier version of the manuscript and Dennis Ross-Degnan, who first stimulated the first author to use the WHO/HAI methodology to measure medicine availability, prices and affordability in Brazil.
As noted above, when first stimulated, most secrete IgM.
In 2000, he revealed the first stimulated emission depletion (STED) microscope images of an E coli bacterium.
The researchers first stimulated the thalamus and identified 190 individual nerve cells in the lamina 1 tract.
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