Sentence examples for we first inserted from inspiring English sources

In order to evaluate the capacity of insulators to block insertional mutagenesis, we first inserted cassette 234-β-EGFP on chromosome 4 at site RL5 to determine if insertion of an expression cassette would activate the flanking genes.

To develop lentiviral vectors that report on Wnt signaling and allow discrimination between uninfected and non-responding cells, we first inserted an SV40-mCherry [23] selection cassette downstream of the 7xTcf-eGFP reporter cassette [20] (7TGC virus, Fig. 1A, top).

To construct expression plasmids encoding the Rnq-polyQ fusion proteins, we first inserted htt exon 1 PCR fragments with 19, 54 and 91 CAG repeats into the NotI and EcoRI sites of pYex2T/L, thereby creating plasmid intermediates.

To construct the LV-1 × SYN-EGFP shuttle vector pTYF-1 × SYN-EGFP, we first inserted the NotI/ ClaI PCR fragment of WPRE amplified from woodchuck hepatitus B virus genomic DNA (NCBI access no: J04514) into the pTYF-SW-linker.

To assess the effect of three copies of the cyb-3 gene, we first inserted a copy of the cyb-3 gene on chromosome IV, in the cxTi10882 Mos1 integration site, and created the dotSi110 stable insertion in addition to the previously generated dotSi100 stable insertion on chromosome II (Tarailo-Graovac et al. 2010).

It was in a boxing ring, of course, that Ali first inserted himself into the consciousness of the British people.

For low implantation doses, Mo first inserted into austenite cells inducing austenite lattice expansion.

They first inserted each enhancer into embryonic mice to learn whether it really did turn genes on.

Fouchier's team first inserted several mutations they knew might help the virus adapt for mammalian spread.

He first inserted that package in the 2012 Budget Resolution, which was adopted by the Republican-controlled House of Representatives.

Multi-gene sequences were first inserted into the pcDNA vector (Invitrogen, Carlsbad, CA, USA).