We first chose to encapsulate an RNA polymerase called polynucleotide phosphorylase (Chakrabarti et al., 1994).
Methods: We first chose the target sequences for siRNA along the coding region of S antigen of HBV adw strain (HBsAg).
As a proof-of-principle study, we first chose Cdx2 and Gsk3α loci as our induced chromosomal translocation target sites for the following reasons: 1. Cdx2 and Gsk3α sgRNAs have been proven to work efficiently in mESCs in our previous experiments.
To estimate water consumption due to population increase, we first chose the districts in Kandahar Province that would most likely be using water from karezes, based on geology (Doebrich and Wahl 2006; Quaternary alluvium preferred), degree of urbanization (Google Earth; rural areas preferred), and proximity to surface water (Google Earth; proximity not preferred).
To identify DMEs, we first chose all regions with STAMP signal greater than a threshold value.
To determine whether differentiation decreases sensitivity of stem cells to NK cell mediated cytotoxicity we first chose to concentrate on hMSCs.
We first chose Cdk2 as a primary candidate, since published data suggested that Akt is involved in the regulation of Cdk2 in an isoform-specific manner [16], [17].
In order to study whether IL-8-producing tumors would retain intratumorally DC injected inside the lesion, we first chose the HT29 xenografts because of their higher bioproduction of IL-8.
We first chose to include the proportion of non-motile clones in the analysis, as we shown previously that this traits has rapidly evolved in some of the selected lineages (see figure 3).
Therefore, we first chose to produce in vitro the leader RNA (47 nt long) from permeabilized VSV virions, since it is biochemically indistinguishable from that isolated from VSV infected cells [15], [16].
We first chose to clone pre-miRNAs for some of the miRNAs that were highly expressed in T cells and where all the clones of mature miRNAs had identical 5' ends (did not show 5' end variations).
