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For complementation of GCP-WD-depleted cells with exogenous GCP-WD and GCP-WD mutants we expressed from a single plasmid shRNA targeting endogenous GCP-WD and RNAi-insensitive EGFP-tagged GCP-WD or GCP-WD mutants.
To test the function of the dibasic element close to the C-term we expressed from a transgene a variant Sosie::Venus, in which the C-terminal …RKHF sequence motif was replaced by …LNHF.
A CDPK1-GFP fusion protein that we expressed from the cdpk1 promoter within the cdpk1 chromosomal locus was present at all life-cycle stages, including the gametocyte, the zygote, and the early oocyst, neither of which possess a functional motor complex.
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A recombinant protein termed PIP (phospholipase inhibitor from python), which we have expressed from the liver of a nonvenomous snake, Python reticulatus [ 17], exhibits in vivo anti-inflammatory activity that correlates well with its in vitro inhibitory potency towards sPLA2.
High level expression of the C46 fusion inhibitor is required for a potent antiviral effect [35], so we expressed C46 from the strong SFFV promoter and expressed MGMT from an IRES.
To investigate the instability of ScPex3mutA+C, we expressed it from the GAL1 promoter and performed a pulse-chase experiment.
Because the chromosomally-encoded HflD is expressed at a very low level and hard to detect, we expressed HflD from a multicopy plasmid.
Because the chromosomally encoded HflD was expressed at a very low level and was difficult to detect, we expressed HflD from a multicopy plasmid.
We expressed Atg36 from a centromeric plasmid either untagged or with an N- or a C-terminal GFP or mRFP tag under control of the GAL1 promoter.
In our previous studies we expressed CLB3 from the GAL1-10 promoter driven by an estrogen inducible Gal4-ER fusion (Carlile and Amon, 2008).
To screen a panel of biogenic and trace amines, we expressed Orco from Culex quinquefasciatus (Southern House Mosquito) in Xenopus oocytes and recorded ligand-induced current responses using two-electrode voltage clamp electrophysiology (see Methods).
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