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To address this, we expressed each of the eight isoforms or different constructs of isoform 1 in Opa1−/− MEFs.
To determine whether nucleolin interacts with other ErbB receptors family members we expressed each of the ErbB proteins with or without Myc-tagged Nucleolin in COS7 cells.
To determine the cellular localization of each mutant, we expressed each in HeLa cells and visualized localization with indirect immunofluorescence (Figure 3C).
To identify mutant Gap1 proteins that might be resistant to down-regulation by Am, we expressed each of the 38 active mutant proteins (i.e. those able to mediate D-His incorporation on proline medium) in gap1Δ cells and compared the growth phenotypes obtained on Am medium with or without D-His.
For visual display (Fig. 1A; Fig. S1; Dataset S1), we expressed each secreted protein level as the log2 fold-change relative to a baseline derived from the average signal for that protein across all samples (all cell strains, growth conditions and oxygen concentrations).
We expressed each of the HA-tagged steroid receptors in combination with each of the seven FLAG-tagged TPR proteins and performed co-immunoprecipitations with antibodies directed against the HA-tagged receptors or the FLAG-tagged TPR proteins, respectively, and visualized co-precipitated proteins by Westernblot analysis.
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In addition, we express each of them in terms of Bernoulli functions.
We express each of u i, i = 1, 2, 3, in terms of wavelet coefficients.
Moreover, we express each of them in terms of Bernoulli functions.
To this end, we express each ({g}'_{ij} ) in terms of parameters and delay.
Thus, we used an orthogonal deviation, in which we express each observation as a deviation of average future observations.
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