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When viewed with a compound microscope we experienced no difficulty in observing GFP and little problem with background fluorescence from CeMM.
Although this may have theoretically reduced the accuracy of the ImageJ methodology in our study, we experienced no difficulties visualising particulate matter within the macrophages and still found ImageJ to be the more reliable of the two methods for detecting PM.
We experience no such difficulties with either our GFP-tagged (N- or C-terminal) or BAP-tagged proteins.
This kind of explanation can certainly help us feel better when we experience difficulty.
However, we experienced difficulty in recruiting men to the focus groups.
This strategy was largely successful, although we experienced difficulty in recruiting men into the focus groups.
We experienced technical difficulties in four patients undergoing reconstruction nailing.
We experienced difficulties in evaluating this item as few studies mentioned uninterpretable results.
After 1- or 2-day intensive training sessions on field data collection, field workers without PDA experience, we find, have no difficulty in conducting PDA-based questionnaires.
We also experienced some difficulty recruiting GPs into the study.
Finally, we have experienced difficulty in predicting the emergence of resistance.
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