We exclusively considered human tissues, because cell lines had originally been introduced as an internal check.
Once a threshold was determined we exclusively considered the positions in the assemblies above the threshold as follows.
Since we exclusively considered the promoter region for shuffling, we thereby preserved the proportion of all the nucleotides in the shuffled sequences as that in the promoter datasets.
Moreover, in order to avoid false positives, we exclusively considered those mutations that were homozygous in at least the two animals of the local breed.
In order to avoid to take into account apparently significant relationships between drug sensitivity and polymorphic variations, which would result from chance only, we exclusively considered as relevant the differences in mean IC50 values of at least 50% (IC50 ratios >1.5 or <0.67) (biological significance) and giving a P-value <10−4 (statistical significance).
We shall exclusively consider second-order PDEs, and hence ( H 0 1 ν ⊂ X ⊂ ( H 1 ν, where ν = 1 (respectively, ν = d ) for a scalar (respectively, vector) field; here L 2 is the space of square integrable functions over Ω, H 1 = { v | v ∈ L 2 , ∇ v ∈ ( L 2 d }, H 0 1 = { v ∈ H 1 : v | ∂ Ω = 0 }.
In a second round, we then exclusively consider the mutations not yet covered, some of which (in conjunction) could point to a different haplogroup affiliation and thus serve as positive evidence for the existence of a secondary constituent.
However, because we are exclusively considering a neutral scenario, we have fixed our population effective size to N = 1000.
We primarily (but not exclusively) consider the p= 2 situation.
