Each plasma sample was double tested, and the obtained values were highly correlated (r = 0.97, N = 83, P < 0.001).
Here we present the behavior of double mutant flies that are trans-heterozygous for cry allele (pdfr5304; ; cryb/01), but we also tested double mutants that were homozygous for the different cry alleles (pdfr5304; ; cryb and pdfr5304; ; cry01) similar to those studied by Zhang et al. [16]: and the results were comparable (Figure 4B, Figure 5B, Data not shown).
To limit this possibility, we also tested double heterozygous combinations of Imd pathway mutations.
We individually tested double mutants of vac17Δ with each of the previously proposed candidates that are not essential genes, and found that the double mutants, vac17Δ pep12Δ and vac17Δ vps45Δ displayed synthetic growth defects.
To overcome this drawback, we propose an improved double test scheme with a fine initialization as well as a suitable search order.
To establish whether β-amyloid and tau pathologies affect hnRNP A/B expression in vivo, we tested double transgenic APPsw/PS1ΔE9 mice and mice expressing dually mutated K257T/P301S Tau for hnRNP A/B alterations.
We tested double mutants, including plx-1 ; unc-129 (whose individual mutants had the highest penetrance among guidance genes), unc-5 ; unc-129, unc-51 ; unc-129, unc-51 ; unc-5, unc-115 ; egl-15, and vab-1 /+; sax-3 (vab-1 ; sax-3 homozygotes arrest before the stage of glr-2:: gfp expression) (Ghenea et al. 2005).
We further tested genetic double mutants that were null for both mmp1 and dfmr1.
We also tested the double knockout kcs1Δ ddp1Δ strain, which accumulates 1-IP7 but has no 5-IP7 or IP8.
We also tested G137A/P138A double mutant for deacylation function and it also showed no activity like the individual point mutants.
