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To investigate the in vivo function of Porin in Drosophila, we determined to generate porin loss-of-function mutants by mobilizing the P-element G6147 inserted near the porin locus (Figure 1D).
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Of the eight aldehyde tag placements that we screened, only one (Tag B) was determined to generate peptides that were likely to be immunogenic.
She visited five countries in four days, determined to generate as many headlines as possible.
A group of 50, mostly women, was determined to generate excitement for Clinton's campaign for president.
In an interview in his office at Brooke, Colonel Holcomb said he was determined to generate data that would help military and civilian surgeons answer those questions.
Enzymatic cleavage reactions were performed at 30°C for 10 minutes with enzyme concentrations empirically determined to generate single cleavage products.
Initial hits were (a) re-assayed at 30 μM for verification and (b) potency determined to generate an approximate IC50.
Next, we determined whether generated CTLs can be labeled effectively using ferumoxides-protamine sulfate (FePro) complexes for CMRI.
We determined that generating the elongated cell shape requires a large protrusive force at the front (the pressure profile there is positive).
Ultimately, we determined that spheroids generated with NOG+SB+FGF4+Ch+SAG and that were subsequently embedded in Matrigel and expanded in FGF10 gave rise to 'Human Lung Organoids' (HLOs).
The template code can then inspect the properties of that element to determine how to generate code from it.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com