Sentence examples for we decided to amplify from inspiring English sources
We don't have a voice here.' That's when we decided to amplify that voice for them, back in America".
Because the cRNA yield from the 1 μg pooled sample test was low, we decided to amplify the smaller samples.
Therefore we decided to amplify this region to investigate the variability of these region in our patients.
In our EES of the River Seymaz, we decided to amplify only the second half of the SSU rRNA gene, which is generally more conserved both in sequence length and primary structure, in the hope of avoiding negative competition against SSU rRNA gene sequences of unusual length or high divergence.
Therefore, considering the age of our sample, we decided to amplify from ancient samples the trnL- trnF region ranging from site 37 to site 215, namely the region that proved useful to distinguish among the different genera (Quercus, Castanea and Fagus) and also among the main taxa (F. sylvatica, F. orientalis, F. japonica, F. grandifolia) inside the Fagus genus.
As a species-specific signal for abortus or melitensis could not be obtained but B. abortus was suspected, it was decided to amplify a larger DNA segment of the target locus of the real-time PCR, which was specific for the species abortus, the BruAb2_0168 gene (BAbS19_II01580 in vaccine strain S19).
It is likely that this rate could be increased with a more limited subset of targets and more optimisation, but for the purposes of this study we decided to focus on the regions that amplified in a majority of species without optimisation.
After several unsuccessful optimizations with published primers [ 9, 12, 15, 19] to amplify MIRU-VNTR and SSR loci, we decided to carry out in-silico analysis of the template sequences and design new primers.
The problem is likely to be amplified by correlation among parameters, which is why we decided to keep the shape parameter constant and estimate the remaining parameters as other researchers have done.
