In order to confirm the charge interplay between proteins and POMs as the key element in crystal formation, we crystallized the model protein hen egg white lysozyme (HEWL, pI ∼ 11) with Na6[TeW6O24]·22H2O (PDB entry: 4PHI).
To investigate the molecular mechanism of PolB, we crystallized the protein and determined its crystal structure (abbreviated as apo-PolB in this work) using molecular replacement (McCoy et al., 2007) and refined at resolution of 2.15 Å (Table S3).
For this purpose, we crystallized unesterified cholesterol from hot ethanol to obtain cholesterol crystals.
To further correlate the ion distribution in the crystal with trans-enhanced dissociation in electrophysiology experiments, we crystallized the channel with a mutant toxin, Lys27Met.
As part of our screening efforts we crystallized the human HSC-70 ATP binding domain and obtained novel crystal forms in addition to known structures.
To obtain insight into the mechanistic role of UNC-45, we crystallized the full-length protein from C. elegans (107 kDa) and determined its crystal structure at 2.9 Å resolution (Table S1 available online).
"We didn't start Lawrenceville, but we like to think we crystallized and focused it," Stern said, and the table agreed.
Lactobacillus plantarum CECT 748T produces an aggregation-prone glycosidase (LpBgl) that we crystallized previously.
Fast forward, and the 23 became 14 in Year 2, then 10 in Year 3, and we crystallized them into two — culture and offerings.
We crystallized Anbu from Hyphomicrobium sp. strain MC1 in four different space groups and solved the structures by SAD-phasing and Patterson search calculation techniques.
To explore the structural features responsible for this taxon specificity we crystallized natural crayfish trypsin in complex with chemically synthesized SGTI.
