Sentence examples for we cryopreserved from inspiring English sources

We used primary cultures of cell lines A and B for SCNT, and we cryopreserved cell lines C and D in a cell cryopreservation solution (Cellbanker; Mitsubishi Kagaku-Iatron, Tokyo, Japan), and then subcultured them with five passages.

After a 30-min incubation at room temperature, we cryopreserved the whole pups in OCT medium, and cryosectioned sagittal sections (10 μm).

On the basis of the above described data, we cryopreserved these MHC multimers at −20°C, with 5% glycerol and 0.5% BSA, keeping each pMHC specificity separated until cell staining.

So, to address the unknown genetic mutations that we might be interested in testing and to maximize the utility of the biorepository, we cryopreserved lymphocytes for future transformation into cell lines.

Since we used cryopreserved CliniMACS populations for these experiments, we expanded the populations prior to functional characterization.

However, we have observed inhibitory effects if we used cryopreserved cells in pellets containing ≥50% labeled cells, although pellets with 10% labeled cells appeared unaltered (Fig. S3A D).

We also cryopreserved one human iPSC line, CHiPS-A, that we had established in collaboration with University of Geneva (Unger et al., 2009) by transducing human skin fibroblasts with Oct-4, Nanog, Sox2 and Lin28.

We have cryopreserved cynomolgus and human ES cells by the vitrification method, using a chemically-defined dimethyl sulfoxide (Me2SO -free and seruMe2SO -freeum composed of Euro-Collins solution anda base medium and 40% (v/v) ethylene glycol (EG) and 10% (w/v) polyethylene glycol (PEG) as cryoprotectants.

We analyzed cryopreserved PBMC provided by these men at 6 months to one year prior to ART initiation and at 1 and 2 years after that time.

To assess this possibility, we sampled cryopreserved peripheral blood mononuclear cells (PBMC) from 20 HIV controllers, 20 ART-suppressed, and 20 untreated non-controllers, and 34 healthy HIV uninfected controls and compared the frequencies of CD25+CD127dim CD4+ Tregs between groups.

We analysed cryopreserved samples taken before these patients began cART and while they were on effective cART to characterize the residual HIV-1 in the plasma and the monocyte and CD4+ T-cell compartments.