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For the remaining genes, a function could not be assigned even if we considered less stringent E values, and these genes were put in the category of "others".
As a reference frame, we considered less than 1,000 mRNA molecules per 1 ng of total RNA as a low expression level, between 1,000 and 10,000 as a moderate expression level, and >10,000 as a high expression level.
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We strive to maintain an element of flexibility in the A-level combinations we accept, and we do not have a list of subjects that we consider less ideal than others.
Let us examine those traits that we have inherited that we consider less than desirable.
An alternative possibility, which we consider less likely, is that there has been rampant horizontal transmission of the same Wolbachia variants within and among S. invicta populations.
EPIG also identified genes with similar expression profiles in both the liver and kidney, which we consider less likely to be involved in hepatotoxicity.
The positive RT-PCR signals could be caused by detection of viral RNA in the inoculum without challenge virus replication (i.e., RNA derived from degraded viruses), which we consider less likely, because RT-PCR titers increased over several days.
Because independent losses of many introns in several lineages would be expected, we consider less likely the scenario postulating that all the introns common to Pseudendoclonium and Oedogonium were present in the last common ancestor of ulvophyceans and chlorophyceans.
In contrast, the relative abundance of a mutant within a pool we consider less informative, since it may be influenced by a number of additional factors, such as the abundance of a vector in the transfection cocktail, the length of its homology arms, or any local variation in recombination rates.
The non-DOA cases, for which the time of incidence was considered less accurate, we analyzed in relation to daily average exposures.
Because changes occasionally observed early after addition of growth factors were considered less relevant, we focused on barrier disturbance established in the cultures during cultivation for more than 24 h.
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