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In order to compare the human chromosome 8p23.1 DEF region to the orthologous locus in our closest relative, we both employed the chimpanzee (Pan troglodytes, ptr) whole genome shotgun (WGS) working draft (WD, [ 23]) and high quality chimpanzee BAC sequences.
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This took place while we were both employed by the U.S. EPA during 1971 1975.
Our financial commitments were high, a never-ending treadmill, but our incomes, as long as we both remained employed, were enough to meet the demands.
We initially employed both univariate and multivariate analyses.
In order to test the effect of DNA-PK on rAAV replication, we have employed both in vivo and in vitro replication assays.
Here we have employed both of the latter methods to demonstrate low levels of infectious prions in clinically normal, conventional assay-negative white-tailed deer orally exposed 19 months previously to urine and feces from CWD+ deer.
Consequently, we also employed both HeLa and HEK293-T cells.
We therefore employed both age and gender as covariates in subsequent analyses.
We then employed both direct and indirect assays to detect the S. Typhimurium/Dectin-1 interaction.
We further employed both neighbor joining (NJ) and the maximum likelihood estimation (MLE) for phylogenetic reconstruction.
To this end we have employed both one and two dimensional liquid chromatography nanospray ionization tandem mass spectrometry (1D and 2D-LC NSI MS/MS).
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