Sentence examples for we bath from inspiring English sources
In an impassioned statement ahead of the demonstration, the team at KCLSU said: "We work in hospital as part of our training: we bath, feed, administer medication, tend wounds, manage extreme emotions, visit people in their homes, wear our heart on our sleeves, with a smile on our face every day that we go to work.
First, to test for a role of phosphodiesterases in generating PKA transients in RGCs, we bath applied the non-specific PDE inhibitor IBMX (100 µM) to retinal explants and monitored PKA activity using AKAR3.
We bath applied the drug at the relatively low concentrations of 5 µM (n = 5) or 1 µM (n = 4) while monitoring its effect on the currents activated by the voltage clamp protocols described above.
We bath applied either the NO-donor SNP (200 μM) or the membrane-permeable cGMP-analogue Br-cGMP (50 μM).
To understand which synaptic receptors were involved in triggering bursts, we bath applied Gabazine, APV or CNQX individually.
To reduce the excitation in the two neuronal networks, we bath-applied the non-competitive NMDA receptor antagonist 2-amino-5-phosphonopentanoic acid (APV) (final concentration, 20 µM; TOCRIS).
Next, we bath-applied 10 nM, 100 nM, or 10 µM DA for 5 minutes and continued to evoke Up-states via hippocampal stimulation and/or trains of local EPSPs.
To reduce inhibition, we bath-applied the ionotropic GABAA receptors antagonists picrotoxin (PTX, final concentration, 10 µM; Sigma) and bicuculline (final concentration, 10 µM; Sigma) to leech ganglia and to hippocampal dissociated cultures, respectively.
To determine if LILFU waveforms were capable of activating Ca2+ transients, we bath-loaded slice cultures prepared from wild-type mice with the Ca2+ indicator Oregon Green 488 BAPTA-1 AM (OGB-1 AM) and Sulforhodamine 101 (to differentiate between neurons and glial cells) as previously described [30].
To determine whether there was any tonic dopaminergic activity affecting the UP states, we bath-applied either 10 μ m SCH23390 or 200 n m raclopride for 40 min to observe the effect on UDS properties.
