First, we assessed steady-state transcription in whole brain and two more specific brain regions.
We first assessed steady-state levels of cell proliferation in the hippocampal dentate gyrus of adult, 8-week-old TCRα –/– mice, which are characterized by a complete lack of αβ T cells (both CD4 + and CD8 +) due to targeted deletion of the gene encoding the TCRα chain (TCRα –/–).
No studies were found that assessed steady state propulsion, change of direction or tilting the chair.
To expand our validation set, we identified 6 additional primary fibroblast lines (3 IPF and 3 control, which were part of the initial microarray study) that matched the sub-cultivation criteria for the original array analysis and assessed steady state protein levels for PDCD8, CFL2 and FUT10 (this requires substantially fewer cells than is needed for polyribosome preparations) (Figure 4F).
Three categories of partner type were assessed: steady (e.g., spouse, boyfriend); casual (e.g., one-night stand); and anonymous (e.g., someone in the park).
To extend observations in mammalian cells, we assessed the steady-state poly(A) tail length of seven validated miRNA targets [52], [53] in cell lines reported to express both the mRNAs and cognate miRNAs [54].
We assessed the steady-state mRNA levels of four mitochondria-encoded genes, each of which code for one subunit of each of the four complexes of the electron transport chain [23].
To potentially explain the protective effects of WT LRRK2, we assessed the steady-state levels of Dnm1-GFP and FLAG-LRRK2 proteins in cortical cultures under similar conditions.
We assessed the steady-state levels of LC3-II, the best characterized marker of the autophagosome, in TFEB-transfected T40PL cells.
To begin to understand how AtGRP7 may influence the differentially expressed transcripts we assessed their steady-state abundance in the atgrp7-1 T-DNA insertion line that lacks AtGRP7 [ 4].
We assessed the steady-state levels of deacylated mt-tRNALeu UUR) by high resolution northern blot analysis in WT and mt-tRNALeu UUR1 mutant cybyids transiently transfected withighe whole Leuresolution the Cterm domainortherne MTS-Cterm domain.
