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Recognizing the limitations of current gene- or gene set-based methods on heterogeneous samples, we alternatively analyzed transcriptomic changes by using regulatory events.
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Suspension cells were alternatively analyzed by metabolic activity measurements with the XTT cell proliferation kit II (Roche, Mannheim, Germany).
Non-adherent U-937 cells were alternatively analyzed for their ability to form colonies in soft agarose by overlaying them onto 2 ml of 0.4% w/v Sea Plaque agarose (Cambrex, East Rutherford, NJ, USA) on top of 3 ml of a 1% w/v peqGOLD agarose underlayer (PeqLab, Erlangen, Germany), both in complete medium.
Alternatively, analyzing SNP frequency differences alone leads to narrower peaks roughly half this size.
The two procedures thus have different characteristics, allowing users to discover different types of interesting combinatorial associations and to alternatively analyze the data according to their specific needs.
Alternatively, we analyzed if up-regulation of MCP-1 was responsible for the stimulatory effect on HPC.
Alternatively, we analyzed the data by dividing the experimental animals into two groups (anti-estrogen and anti-androgens) by including the two gonadectomized animals into these groups (the female into the anti-estrogen and the male in the anti-androgen group).
Alternatively, we have analyzed the frequency of cobblestone area-forming cells (CAFC) at day 28 to estimate the fraction of more primitive progenitor cells.
Following this reasoning we analyzed alternatively spliced Arabidopsis genes and found that they show the tendency to have a wide expression breadth and longer primary transcript with more and longer introns (data not shown).
As SR proteins, a family of RRM-containing important splicing factors, are known to be extensively alternatively spliced in Arabidopsis, we analyzed alternative splicing at NAGNAG acceptors in SR and SR-related genes.
Alternatively, we can analyze overdispersed data with robust parametric noise models like Student's- t distributions (cf. Gottardo et al., 2006).
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