In order to confirm the validity of our sequences and to test the de novo assembly against a reference assembly, we also assembled the junco transcriptome using GMAP [ 38] with standard parameters against the closest available draft genome assembly: the zebra finch the first passerine genome sequencing project [ 39], and a species that diverged from juncos approximately 25 Ma ago [ 40].
We also assembled 369,092 spliced Arabidopsis transcript alignments into 29,183 alignment assemblies, of which 22,951 include FL-cDNAs (Table 1).
We also assembled VOIs in the medial and lateral occipital areas in this atlas and constructed the Medial_Occipital_VOI and the Lateral_Occipital_VOI.
We also assembled prototype Na-ion full cells, consisting of the as-prepared free-standing Sn@CNT@carbon paper anode and Na0.80Li0.12Ni0.22Mn0.66O2 cathode.
We also assembled a 10 23 catalytic motif containing general purpose RNA-cleaving DNA-enzyme (Dz) against the same region, which cleaved the target RNA very efficiently.
We also assembled minichromosomes in the presence of the H1.4 domain mutants and tested their affinity for chromatin and effect on nucleosome spacing.
We also assembled cluster #1 sequences from a further four kinetoplastid species, and found that all eight kinetoplastid species analysed with MEME gave a variant of [UAGAU] as the best-scoring motif for this cluster (Table S5).
To distinguish between motifs for the SPP and the acetylase, we also assembled those proteins for which the most N-terminal identified peptide was within 10 aa residues from the predicted cTP cleavage site (upstream or down-stream) and for which the N-terminal residue was un-modified.
To confirm that our approach detected multiprotein complexes bound to the DNA, and not merely GST-CSL that was no longer in complex with Notch1 (or MAML-1), we also assembled complexes using hexahistidine-tagged CSL, and prepared a GST-Notch1 fusion protein for use in place of unlabeled Notch1.
We also assembled three 'species' datasets that consisted of all the complete aligned genome sequences from strains of the species Turnip mosaic virus (TuMV), Potato virus Y (PVY) and Plum pox virus (PPV); these datasets were of 61, 41 and 22 sequences respectively.
